茶叶科学2017，Vol.37Issue(1)：86-96,11.
茶树牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS的克隆及表达分析
Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)
摘要
Abstract
A full-length cDNA sequence encoding geranylgeranyl diphosphate synthase (GGDPS) was isolated from transcriptome database of tea plant, cloned from C. sinensis cv. Tieguanyin and named as CsGGDPS. The cDNA length of CsGGDPS was 1661 bp, with an open reading frame (ORF) of 1137 bp and deduced protein of 378 amino acids. The protein was deduced to contain 5 conserved domains with 2 functional domains of Isoprenoid-Biosyn-C1 superfamily. The sequence analysis showed that CsGGDPS was highly conserved and had the closest genetic relationship with Panax notoginseng. CsGGDPS was an instability and hydrophilic protein, which was predicted to be located in chloroplast but with no transmembrane structure and signal peptide. There were 20 phosphorylation sites within the polypeptide chain. Alpha helix was predicted to be the major secondary structure of CsGGDPS. The three-dimension structure of CsGGDPS was highly similar to GGPPS11 from Arabidopsis thaliana. The quantitative real-time PCR showed that CsGGDPS expression was increased during the developmental process and increased with the age of tea leaves. Meanwhile, its expression was also enhanced during the Zuoqing procedure. CsGGDPS was ubiquitously expressed in the C. sinensis cv. Huangdan, cv. Tieguanyin and their first filial generation cv. Jinguanyin, but with different expression levels.关键词
茶树/牻牛儿基牻牛儿基焦磷酸合成酶/茶叶香气/基因表达Key words
Camellia sinensis/GGDPS/tea aroma/gene expression分类
农业科技引用本文复制引用
姚雪倩,岳川,杨国一,陈丹,张冬桃,陈桂信,叶乃兴..茶树牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS的克隆及表达分析[J].茶叶科学,2017,37(1):86-96,11.基金项目
国家自然科学基金(31600555)、福建省"2011 协同创新中心"中国乌龙茶产业协同创新中心专项(闽教科〔2015〕75 号)、福建茶产业农技推广服务试点建设(KNJ-151001). （31600555）
