现代食品科技2017，Vol.33Issue(2)：21-28,34,9.DOI:10.13982/j.mfst.1673-9078.2017.2.004
茶黄素与真菌多糖联合清除ABTS自由基活性的比较
ABTS Radical Scavenging Activity of Theaflavins Combined with Fungal Polysaccharides
摘要
Abstract
The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of theaflavins (TFs) alone and combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP) were compared in this paper.The results of ABTS radical scavenging experiments were analyzed using the Chou-Talalay combination index (CI),and the scavenging effects,combination index (CI),and dose-reduction index (DRI) of TFs,AAP,and GLP,alone and in combination,were evaluated.The half maximal inhibitory concentration (IC50) values ofTFs,AAP,and GLP were 20.60 μg/mL,2.49 mg/mL,and 0.93 mg/mL,respectively,while the IC50 values of a combination of TFs and AAP (mass ratio 1:3) and a combination of TFs and GLP (mass ratio 1∶3) were 74.04 μg/mL and 58.92 μg/mL,respectively.CI analysis indicated that with the clearance rate in the range of 5～97％,the C1 values of TFs with GLP were ＜ 1 and exhibited a downward trend,suggesting a synergistic effect between TFs and GLP.DRI analysis demonstrated that with the clearance rate in the range of 5～97％,the DRI values of TFs with GLP were greater than 1.Inconsistent results were obtained in the above analyses for a combination of TFs and APP.Therefore,there was a synergistic effect between TFs and GLP for scavenging ABTS free radicals,but no similar effect was found between TFs and APP.关键词
真菌多糖/茶黄素/联合/抗氧化Key words
fungal polysaccharides/theaflavin/combination/antioxidant activity引用本文复制引用
张乃珣,尹红力,刘冉,于美汇,李婷婷,王振宇..茶黄素与真菌多糖联合清除ABTS自由基活性的比较[J].现代食品科技,2017,33(2):21-28,34,9.基金项目
国家自然科学基金项目(GA13B202) （GA13B202）
