食品与发酵工业2017，Vol.43Issue(2)：33-38,6.DOI:10.13995/j.cnki.11-1802/ts.201702006
中间代谢产物对白色链霉菌ε-聚赖氨酸合成的影响
Effect of intermediate metabolites on ε-poly-lysine biosynthesis in Streptomyces albulus
摘要
Abstract
Influences of intermediate metabolites,citric acid,L-Aspartic acid and L-Lysine on the biosynthesis of ε-poly-lysine (ε-PL) were studied.Single factor tests indicated that addition of 1.0 g/L citric acid at the start of fermentation and addition of 0.3 g/L L-Aspartic acid and 1.0 g/L L-Lysine at 12 h of fermentation could increase production of ε-PL to 42.5％,28.7％ and 44.1％,respectively.Results of orthogonal test showed that only L-Lysine and citric acid affected the production of ε-PL significantly (P ＜ 0.05),while L-Aspartic acid not.Feeding 1.0 g/L citric acid and L-Lysine into culture would improve production of ε-PL to 60.1％.Results of cell-free system experiment showed that ε-PL was detected only in L-Lysine feeding group,which further suggested that L-Lysine was immediate precursors of ε-PL.Therefore,production of ε-PL could be improved effectively by addition of intermediate metabolites appropriately.关键词
中间代谢产物/ε-聚赖氨酸/生物合成/白色链霉菌Key words
intermediate metabolites/ε-poly-lysine/biosynthesis/Streptomyces albulus引用本文复制引用
赖永勤,曾天府,熊瑞,郭诗琪,刘丹丹,李学如..中间代谢产物对白色链霉菌ε-聚赖氨酸合成的影响[J].食品与发酵工业,2017,43(2):33-38,6.基金项目
四川省科技支撑项目(2012FZ0048)和国家大学生创新创业训练计划项目(批准号:201610613064)资助 （2012FZ0048）
