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细菌纤维素管的发酵条件优化及结构性能研究

汤卫华 贾士儒

食品与发酵工业2017,Vol.43Issue(2):39-43,5.
食品与发酵工业2017,Vol.43Issue(2):39-43,5.DOI:10.13995/j.cnki.11-1802/ts.201702007

细菌纤维素管的发酵条件优化及结构性能研究

Optimization of fermentation conditions for bacterial cellulose tube and study on its structure performance

汤卫华 1贾士儒2

作者信息

  • 1. 天津现代职业技术学院生物工程学院,天津,300350
  • 2. 天津市工业微生物重点实验室,天津,300457
  • 折叠

摘要

Abstract

Acetobacter xylinum are γ-negative,aerobic,rod-shaped bacteria.Bacterial cellulose (BC) tube was produced by fermenting Acetobacter xylinum on inner surface of oxygen-permeable silicone tube.The effects of cuhure medium,fermentation time,and inoculum on the yield of BC tube were studied.The culture medium was complex culture.The maximum BC tube concentration was achieved after culture with 4 % (v/v) inoculums for 6 days.The bacterial cellulose tube was characterized by FTIR and XRD.The crystallinity index of the BC tubes was 82.6 %,which was slightly lower than the value of BC membrane.Tensile strengths of BC tubes were 50.25 MPa,which was close to the value of BC membrane.The morphology of the tubes was characterized by Atomic Force Microscope (AFM).Results revealed that BC tube was synthesized in the form of microfibrils creating highly entangled network of micro-and nanofibers.Compared with BC membrane,microfibrils of BC tubes had significant differences in geometrical dimensions.This may be able to explain why the crystallinity and tensile properties of BC tube were slightly lower than BC membrane.The experiment results can provide basic data for the application of BC tube on high value-added food packaging materials and tissue engineering scaffolds.

关键词

细菌纤维素管/发酵条件/优化/性能表征

Key words

bacterial cellulose tube/fermentation condition/optimization/structure performance

引用本文复制引用

汤卫华,贾士儒..细菌纤维素管的发酵条件优化及结构性能研究[J].食品与发酵工业,2017,43(2):39-43,5.

基金项目

国家自然科学基金(21106105) (21106105)

食品与发酵工业

OA北大核心CSCDCSTPCD

0253-990X

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