食品与发酵工业2017，Vol.43Issue(2)：44-48,5.DOI:10.13995/j.cnki.11-1802/ts.201702008
大豆低聚肽中抗氧化肽的分离纯化及结构鉴定
Separation, purification and structural identification of antioxidant peptides derived from soybean oligopeptides
摘要
Abstract
In order to investigate the activity and structure of antioxidant peptides derived from soybean oligopeptides (SOP),SOP was prepared by two-step enzymolysis.On the basis of analysis of compositions,the antioxidant activity of SOP was evaluated by DPPH radical scavenging activity.The results showed that the half-inhibition concentration (IC50) of DPPH radical scavenging activity of SOP was approximately 2.6 mg/mL.Then,SOP was separated and purified by reversed phase high performance liquid chromatography (RP-HPLC).The DPPH radical scavenging activity was evaluated in 6 collected fractions.The results indicated that all fractions had higher DPPH radical scavenging activity than SOP.Finally,Q-TOF mass spectrometer was applied in identification of peptides in fraction # 5 and its DPPH radical scavenging activity of 6 identified peptides was analyzed.The results indicated that all the peptides exhibited DPPH radical scavenging activity and Leu-Tyr (LY),Leu-Ala-Gly-Arg (LAGR) and Phe-Ser-Arg (FSR)showed stronger activities than SOP.And they allhad higher antioxidant capacity.关键词
大豆低聚肽/抗氧化肽/分离纯化/结构鉴定Key words
soybean oligopeptides/antioxidant peptides/separation and purification/structural identification引用本文复制引用
刘文颖,谷瑞增,鲁军,潘兴昌,蔡木易..大豆低聚肽中抗氧化肽的分离纯化及结构鉴定[J].食品与发酵工业,2017,43(2):44-48,5.基金项目
国家十三五重点研发计划(2016YFD0400604) （2016YFD0400604）
国家高技术研究发展计划(863计划)项目(2013AA102205-02) （863计划）
