食品与机械2017，Vol.33Issue(1)：16-21,6.DOI:10.13652/j.issn.1003-5788.2017.01.004
产β-苯丙氨酸变位酶基因工程菌的构建及其发酵条件优化
Construction of recombinant E coli.of producing β-phenylalanine aminomutase and optimization of fermentation conditions
摘要
Abstract
The genetic engineering strain with recombinant plasmid pET-28a-pam was expressed in E.coli BL21.The fermentation conditions of initial pH, shaking speed, inoculation time and fermentation time were optimized with the PAM activity by using single factor.The results showed that the optimal fermentation conditions were initial pH 7.0, 200 r/min, the tenth hour broth and liquid volume 50 mL/500 mL, fermented for 24 h.Furthermore, the three factors were optimized by Box-Behnken design, and the results showed that inoculation time, initial pH and fermentation time are main affecting factors and their optimal levels are 12.8 h, 6.7 and 25.2 h.Under the optimal conditions, th activity of PAM reached 11.15 U/mL, improved by 128.9% compared with that under initial culture conditions.关键词
重组大肠杆菌/产酶条件/β-苯丙氨酸变位酶/发酵Key words
Recombinant E coli./Enzyme production conditions/β-phenylalanine aminomutase/fermentation引用本文复制引用
葛飞,唐尧,朱龙宝,李艺雯,段振超,马琪森,陶玉贵..产β-苯丙氨酸变位酶基因工程菌的构建及其发酵条件优化[J].食品与机械,2017,33(1):16-21,6.基金项目
国家自然科学基金面上项目(编号:31671797) （编号:31671797）
安徽省高等学校省级自然科学研究重点项目(编号:KJ2016A801) （编号:KJ2016A801）
国家级大学生创新创业训练计划项目(编号:201510363072) （编号:201510363072）
