食品与机械2017，Vol.33Issue(1)：143-147,5.DOI:10.13652/j.issn.1003-5788.2017.01.033
赤灵芝多糖分离纯化及体外抗氧化性研究
Study on isolation and antioxidant activity of the polysaccharide from Ganoderma lucidum
摘要
Abstract
Isolation, purification and antioxidant activity in vitro of polysaccharide from Ganoderma lucidum were studied.Polysac-charide was extracted from G.lucidum, and three fractions were isolated through ion exchange chromatography and allyl dextran gel chromatography from them, including GLPa-2, GLPb-1, and GLPc.The weight-average molecular weight was detected by gel permeation chromatographic, and then monosaccharide composition was detected by gas chromatography.The results were described as follows.The component parts of polysaccharides were composed of glucose, arabinose, xylose and mannose, with different containing rates, and the average molecular weight of GLPa-2, GLPb-1, GLPc was detected to be 3.65×105 Da, 3.87×104 Da, 1.38×104 Da, respectively.Moreover, a dose-effect relation between the concentration of polysaccharides fraction and the eliminating ratio was also found, and GLPa-2, composed of glucose, arabinose, xylose, and mannose, the largest molecular among the three ones, showed the highest antioxidant activity.Thus it turned out that the antioxidant activity of polysaccharides from G.lucidum was related to their composition.关键词
赤芝多糖/分离/分子量/单糖组成/抗氧化Key words
Ganoderma lucidum polysaccharides/isolate/molecular weigh/monosaccharide composition/antioxidant activity引用本文复制引用
徐雪峰,李桂娟,闫浩,张玉,张敬畅..赤灵芝多糖分离纯化及体外抗氧化性研究[J].食品与机械,2017,33(1):143-147,5.基金项目
海南省自然科学基金项目资助(编号:214034) （编号:214034）
