食品与机械2017，Vol.33Issue(1)：167-171,5.DOI:10.13652/j.issn.1003-5788.2017.01.038
超声辅助提取兔皮胶原蛋白及其理化特性
Ultrasonic-assisted extraction and physicochemical characteristics of collagen from rabbit-skin
摘要
Abstract
The application of ultrasonic irradiation to extract pepsin-soluble collagen from the skins of rabbit was investigated in this study.The results showed that the extraction rate of collagen using the ultrasonic irradiation was (83.69±0.51) % and the yield increased by 25% in comparison with the conventional pepsin isolation method.The result of polyacrylamide gel electrophoresis confirmed that the subunits structure of collagen using the ultrasonic irradiation was mainly of α1, α2 and β, without subunit degradation.Moreover, the ultraviolet spectrum exhibited a typical absorption peak at 217 nm, indicating that it greatly maintained the characteristics of type I collagen.Additionally, the Fourier transform infrared spectroscopy revealed that hydrogen bond was partially destroyed, but the triple helix structure of collagen remained intact even after the ultrasonic irradiation.However, the result of Scanning Differential Calorimeter analyses showed that the thermal denaturation temperature and enthalpy value of collagen using the ultrasonic irradiation were slightly lower than that of the traditional rabbit collagen, and it still had a relatively higher thermal stability.关键词
兔皮/胶原蛋白/超声波/理化特性Key words
rabbit skin/collagen/ultrasonic/physicochemical characteristic引用本文复制引用
张晓洁,张宇昊,马良,马明思,郑红,孙艺..超声辅助提取兔皮胶原蛋白及其理化特性[J].食品与机械,2017,33(1):167-171,5.基金项目
国家自然科学基金项目(编号:31301425) （编号:31301425）
中央高校基本科研业务费重大项目(编号:XDJK2015A015) （编号:XDJK2015A015）
中国博士后科学基金面上项目(编号:2014M562267) （编号:2014M562267）
中国博士后科学基金特别资助项目(编号:2015T80951) （编号:2015T80951）
重庆市基础科学与前沿技术研究重点项目(编号:cstc2015jcyjBX0116) （编号:cstc2015jcyjBX0116）
第四批重庆市高等学校优秀人才支持计划 （）
