中国食品学报2017，Vol.17Issue(2)：10-16,7.DOI:10.16429/j.1009-7848.2017.02.002
马尾藻岩藻聚糖硫酸酯对高脂血症小鼠脂质代谢相关酶的影响
The Regulatory Effect of Sargassum Fucoidan on the Lipid Metabolism Related Enzymes and Cholesterol Synthesis Key Enzyme of Hyperlipidemic Mice
摘要
Abstract
Objective:The objective of this study was to examine the effects of different molecular weights of sargassum fucoidan on the lipid metabolism related enzymes and cholesterol synthesis key enzyme of hyperlipidemic mice.Method:Hyperlipidemic mouse models were established by giving mice medium molecular weight fractions (MMWF) and low molecular weight fractions (LMWF) of sargassum fucoidan by gavage in vivo.Measurements were performed in both the serum and liver to determine lipid metabolism related enzymes and cholesterol synthesis key enzyme indexes,including serum lecithin cholesterol acyltransferase (LCAT),hepatic lipase (HL),lipoprotein lipase (LPL) and liver LCAT,LPL activity.The effect of MMWF and LMWF on the indexes of lipid metabolism was explored.Results:All 4 Sargassum fucoidans were able to increased LCAT,HL and LPL activities,but decreased HMG-COA activity.Compared with the model group,the high-dose MMWF1 and MMWF2 groups,the serum LCAT activities were increased by 17.65％,27.21％,10.97％,10.51％,liver LCAT activities were increased by 39.73％,43.44％,27.72％ and 32.22％;the serum HL activities were increased by 19.70％,25.76％,8.08％ and 19.19％,but the effects on liver HL activity was not significant;the serum LPL activities were increased by 47.16％,51.14％,39.77％ and 44.89％,the liver LPL activities were increased by 47.16％,51.14％,39.77％ and 44.89％,40.22％,50.00％,31.52％,36.96％;the serum HMG-CoA activities were decreased by 36.70％,38.39％,31.20％ and 33.59％,the liver HMG-CoA activities were decreased by 18.63％,22.07％,14.54％ and 17.01％. Conclusions:All 4 Sargassum fucoidans were able to significantly increase serum lecithin cholesterol acyltransferase (LCAT),hepatic lipase (HL),lipoprotein lipase (LPL) and liver LCAT,LPL activity,increasing the key enzyme of lipoprotein metabolism to accelerated the catabolism of TC and LDL,or by inhibiting cholesterol synthesis enzyme HMG-CoA reductase to decrease the cholesterol synthesis.关键词
围氏马尾藻/岩藻聚糖硫酸酯/高脂血症/脂质代谢Key words
Sargassum wightii/fucoidan/hyperlipidemia/lipid metabolism引用本文复制引用
谌素华,王维民,蔡璐,钟思燕,钟赛意,谢恩义..马尾藻岩藻聚糖硫酸酯对高脂血症小鼠脂质代谢相关酶的影响[J].中国食品学报,2017,17(2):10-16,7.基金项目
广东省自然科学基金项目(2015A030313618) （2015A030313618）
湛江市科技计划项目(2014A03017) （2014A03017）
