中国食品学报2017，Vol.17Issue(2)：212-219,8.DOI:10.16429/j.1009-7848.2017.02.028
沙门氏菌血清型快速PCR鉴定方法的建立
Development of Rapid PCR Determination Method of Salmonella Serovars
摘要
Abstract
In this study,78 Salmonella strains were detected into 21 serovars by using traditional slide agglutination test,mainly representing S.Typhimurium and S.Enteritidis.A straincoded as SJTUF10023 was proved to be self-curing,which led to the failure of serovar deternination by slide agglutination test.MLST was used to predict different serovars,and it turned out that each clustering branch was substantially corresponded with one single serovar (96.2％,75/78).However,three exceptions were found in one S.Agona,one S.Aberdeen and one S.Stanley.According to the molecular basis of serovars,O antigen (rfb gene cluster),H1 antigen (fliC gene) and H2 antigen (filjB gene),PCR reactions were designed and combined with DNA sequencing to serotyping the 78 Salmonella strains.The accuracy was as high as 98.7％ (77/78) with the only one exception that happened to the false positive result of S.TyphiH2 antigen;moreover,the self-curing Salmonella SJTUF10023 was identified as S.Typhimurium.The results demonstrated that the PCR determination method established in this study can accurately and rapidly detect Salmonella serovars,which is promising to be an alternative to traditional serotyping methods of Salmonella.关键词
沙门氏菌/血清型/MLST/PCR检测Key words
Salmonella/serotyping/MLST/PCR detection引用本文复制引用
方婷子,史贤明,施春雷..沙门氏菌血清型快速PCR鉴定方法的建立[J].中国食品学报,2017,17(2):212-219,8.基金项目
国家863课题(2012AA101601) （2012AA101601）
国家科技支撑计划课题(2012BAK17B10) （2012BAK17B10）
上海市国际合作项目(14390711900) （14390711900）
