中国食品学报2017，Vol.17Issue(2)：220-226,7.DOI:10.16429/j.1009-7848.2017.02.029
单核细胞增生李斯特菌食品分离株的分子分型鉴定
Characterization of Listeria monocytogenes Isolates from Foods by Molecular Subtyping
摘要
Abstract
Listeria monocytogenes is a major foodborne pathogen,which generally causes human illness including meningitis,meningoencephalitis and abortion.In this study,our aim was to characterize a total of 86 L.monocytogenes food isolates in Shanghai by serotyping and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) subtyping analyses.All the isolates were serotyped by multiplex PCR assays into three serogroups:1/2a or 3a (72.1％,n =62),1/2b or 3b or 7 (19.8％,n =17) and 1/2c or 3c (8.1％,n =7).At the relative similarity coefficient of 0.80,86 isolates and 4 reference strains were identified into 10 clusters and 8 singletons by ERIC-PCR.It was found that the L.monocytogenes isolates sharing the same serogroup could be clustered together by ERIC-PCR at a certain relative similarity coetficient,while isolates belonging to the same serogroup could be efficiently distinguished according to diverse ERIC-PCR profiles.Furthermore,our results will provide the fundamental data for epidemiological surveillance of L.monocytogenes.关键词
单核细胞增生李斯特菌/食品分离株/ERIC-PCR/血清型Key words
Listeria monocytogenes/food isolates/ERIC-PCR/serotype引用本文复制引用
王文凯,陈万义,索玉娟,周秀娟,施春雷,史贤明..单核细胞增生李斯特菌食品分离株的分子分型鉴定[J].中国食品学报,2017,17(2):220-226,7.基金项目
政府间国际科技创新合作重点专项(2016YFE0106100) （2016YFE0106100）
