茶叶科学2017，Vol.37Issue(2)：149-159,11.
茶树磷酸烯醇式丙酮酸转运子CsPPT2基因的克隆和分析
Cloning and Expression Analysis of Phosphoenolpyruvate Transporter Gene CsPPT2 in Tea Plant(Camellia sinensis)
摘要
Abstract
A full length cDNA sequence of phosphoenolpyruvate transporter gene (CsPPT2) was obtained from tea plant(Camellia sinensis) cultivar 'Baiye 1' by polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR(RACE-PCR). The nucleotide sequence length of this gene was 1469 bp, containing a complete open reading frame (1218 bp) encoding 406 amino acids. Bioinformatic analysis showed that the predicted molecular weight of the protein was 44.6 kD, and the theoretic isoelectric point was 9.90. CsPPT2 has 6 trans-membrane regions, which may belong to the hydrophobic chloroplast trans-membrane protein. Phylogenetic tree analysis showed the phosphoenolpyruvate transporter family in tea plant could be divided into two subgroups, with CsPPT1 and CsPPT2 belonged to different subgroups. The expression of CsPPT1 and CsPPT2 were compared in different periods and plant organs. It showed that CsPPT2 was expressed in all tested tissues, with higher expression level in roots and mature leaves than those of CsPPT1. But the expression level of CsPPT1 in young shoots was higher than CsPPT2. CsPPT2 was transiently elevated at the beginning of the albino, but inhibited during the development of albino, indicates that the inhibition of the expression of CsPPT2 may act as a key factor for low-catechins and high-amino acids in 'Baiye 1'.关键词
茶树/白叶1号/磷酸烯醇式丙酮酸转运子/CsPPT2/表达分析Key words
Camellia sinensis/Baiye 1/phosphoenolpyruvate transporter/CsPPT2/expression analysis分类
农业科技引用本文复制引用
纪志芳,甘玉迪,陈常颂,杨鼎俊,孙康,黎星辉,陈暄..茶树磷酸烯醇式丙酮酸转运子CsPPT2基因的克隆和分析[J].茶叶科学,2017,37(2):149-159,11.基金项目
现代农业产 业技术体系建 设专项资 金 ( CARS-23)、江苏 高校优势 学科建设 工程资助项目 、国家自 然科学基 金(31570691)、江苏省科技支撑计划(BE2009313-1) （ CARS-23）
