肉类研究2017，Vol.31Issue(4)：36-42,7.DOI:10.7506/rlyj1001-8123-201704007
动物源性食品中喹诺酮类药物残留的检测
Screening and Confirmation of Quinolones Residues in Animal-Derived Food
摘要
Abstract
A new microbial chromogenic assay was developed to analyze antibiotic residues in animal-derived food and after that, the positive samples were identified by a high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS) method. E.coli was used as the indicator strain to produce a 96-well microplate for detecting 15 kinds of quinolones. Muscle samples were extracted by pH 7.4 phosphate-acetonitrile buffer. This system of microbial chromogenic assay consisted of 150 μL the reaction solution, 50 μL of bacterial suspension (the initial A600 nm value was around 0.4), and 100 μL of the extract solution. The assay proved to be simple and cheap and the results were accurate and easy to interpret. The detection limits for 15 kinds of quinolones drugs were 40-200 μg/kg, meeting the domestic and international requirements for the determination of antibiotic residue limits. Consistent results were obtained for 50 samples of animal-derived food by microbial chromogenic assay and HPLC-MS, indicating that the assay could be used to detect antibiotic residues in animal-derived food with good stability.关键词
微生物显色法/高效液相色谱-质谱法/喹诺酮类药物/动物源性食品Key words
microbial chromogenic assay/high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS)/quinolones/animal-derived food分类
轻工纺织引用本文复制引用
范维,高晓月,陈超,李贺楠,李莹莹,郭文萍,陈淑敏..动物源性食品中喹诺酮类药物残留的检测[J].肉类研究,2017,31(4):36-42,7.基金项目
"十二五"国家科技支撑计划项目(2014BAD04B05) （2014BAD04B05）
