食品工业科技2016，Vol.37Issue(11)：58-61,66,5.DOI:10.13386/j.issn1002-0306.2016.11.003
壳聚糖季铵盐对小牛胸腺DNA的作用研究
Study on the interaction between quaternary ammonium salt of chitosan and calf thymus DNA
摘要
Abstract
2-Hydroxypropyltrimethyl ammonium chloride chitosan(HTCC)were synthesed in heterogeneous system and characterized by IR and NMR spectroscopy.HTCC was the product that the N-H of CS located at C2was replaced by quaternary ammonium salt.In the physiplogical environment(pH =7.4).The interaction of HTCC with calf thytus DNA(ctDNA)were performed by fluorescence spectra and circular dichroism spectra,using acridine orange(AO) as a molecular probe.The analysis of UV Vis and fluorescence spectra showed that there was strong interaction between HTCC and ctDNA,the addition of HTCC made the fluorescence quenched effectively,which fitted the characteristics of the static fluorescence quenching.The circular dichroism spectra and thermal denaturation experiment proved that they combined with each other through the intercalative binding mode.关键词
壳聚糖季铵盐/小牛胸腺DNA/光谱法/结合方式/猝灭机理Key words
quaternary ammonium salt of chitosan/calf thymus DNA/spectral method/combination mode/quenching mechanism分类
轻工纺织引用本文复制引用
齐炎,夏彩芬,胡帆,卢壵,黄梦霞..壳聚糖季铵盐对小牛胸腺DNA的作用研究[J].食品工业科技,2016,37(11):58-61,66,5.基金项目
国家自然科学基金项目(21503075) （21503075）
湖北省教育厅科学技术研究项目(B2015034)资助. （B2015034）
