食品工业科技2016，Vol.37Issue(11)：85-88,4.DOI:10.13386/j.issn1002-0306.2016.11.009
光裸方格星虫纤溶活性蛋白的提取及抗氧化活性研究
Extraction and antioxidant activity of the protein fraction with fibrinolytic activity from Sipunculus nudus Linnaeus
摘要
Abstract
Objective:To extract the protein fraction with fibrinolytic activity from Sipunculus nudus Linnaeus and evaluate the antioxidant activity.Methods:Fresh Sipunculus nudus were precipitated with PBS buffer(0.02 mol/L,pH7.5) to homogenize and the clear supernatant of tissue homogenate was salting out with stepwise ammonium sulfate to obtain the protein fraction with highly fibrinolytic enzyme activity.The antioxidant activity was investigated by using methods of purssian blue reaction,DPPH,phenanthroline and pyrogallol autoxidation.Result:The protein fraction with highly fibrinolytic enzyme activity was obtained by using 30％ saturation ammonium sulfate to remove the mixed protein,and then by 50％ saturated ammonium sulfate to obtain the target protein precipitation.The protein fraction had reduction ability and strong scavenging effect on DPPH· and · OH.The concentration of scavenging 50％ (EC50) of DPPH · and · OH were 1.23 mg/mL and 6.07 mg/mL respectively.Whereas it had significant negative scavenging ability on O2-·.Conclusion:The protein fraction with fibrinolytic activity from Sipunculus nudus Linnaeus obtained by two steps ammonium sulfate salting out method had certain antioxidant capacity.关键词
方格星虫/提取/纤溶活性/抗氧化Key words
Sipunculus nudus Linnaeus/extraction/fibrinolytic enzyme activity/antioxidant activity分类
轻工纺织引用本文复制引用
李映新,李肖肖,刘金远,姚佩珊,颜池,雷丹青..光裸方格星虫纤溶活性蛋白的提取及抗氧化活性研究[J].食品工业科技,2016,37(11):85-88,4.基金项目
广西壮族自治区教育厅专利资助项目(ZL2014008) （ZL2014008）
广西医科大学青年基金(GXMUYSF2014025) （GXMUYSF2014025）
广西高校/广西再生医学重点实验室开放课题项目(桂再重开15-05). （桂再重开15-05）
