食品工业科技2016，Vol.37Issue(11)：113-117,5.DOI:10.13386/j.issn1002-0306.2016.11.015
胆盐水解酶基因在植物乳杆菌ST-Ⅲ中的表达
Gene expression of the bile salt hydrolase in Lactobacillus plantarum ST-Ⅲ
摘要
Abstract
Bile salt hydrolase(BSH),the enzyme deconjugating bile potentially plays an important role in reduction of blood cholesterol level,which has received a widespread attention.In this study,the recombinant Lactobacillus plantarum ST-Ⅲ were constructed by overexpressing four BSH genes and the BSH enzyme activities were determined.The result showed that L.plantarum ST-Ⅲ harboring BSH1 had a higher enzyme activity than the other three strains,then was the strains harboring BSH3 and BSH2,and the lowest activity was shown in ST-Ⅲ overexpressing BSH4,which indicated that the BSH1 might play a more important role in the hydrolysis of the conjuncted bile salts,then was BSH3,BSH2 and BSH4 successively.关键词
胆盐水解酶/植物乳杆菌ST-Ⅲ/克隆表达/酶活性Key words
Bile salt hydrolase/Lactobacillus plantarum ST-Ⅲ/gene clone and overexpression/enzyme activity分类
轻工纺织引用本文复制引用
黄艳娜,毕雪威,游春苹..胆盐水解酶基因在植物乳杆菌ST-Ⅲ中的表达[J].食品工业科技,2016,37(11):113-117,5.基金项目
国家“十二五”科技支撑计划(2013BAD18B01,2013BAD18B02). （2013BAD18B01,2013BAD18B02）
