食品工业科技2016，Vol.37Issue(11)：134-140,146,8.DOI:10.13386/j.issn1002-0306.2016.11.020
乳酸菌CRISPR-Cas系统同源性分析
Homology analysis of CRISPR-Cas system in Lactic acid bacteria
摘要
Abstract
To analyze the homology CRISPR-Cas systems in Lactic acid bacteria.Bioinformatics method was used for predicting CRSPR-Cas system of Lactic acid bacteria that published in NCBI.There phylogenetic analysis was performed to investigate the level of conservation and divergence between the identified Lactic acid bacteria CRISPR immune systems using the repeats and universal Cas protein.Streptococcus thermophilus,Lactobacillus delbrueckii subsp.bulgaricus and Lactobacillus helveticus all contain different number of CRISPR-Cas systems,the repeats of each type were highly conserved and the Cas proteins demonstrated high similarity.For homology analysis,repeats and Cas proteins showed high similarity with several individual species of distant relationships,which suggested that the CRISPRs of tested strains also had the characteristic of horizontal gene transfer(HGT).关键词
乳酸菌/clustered regularly interspaced short palindromic repeats(CRISPR)-Cas系统/同源性/重复序列/间隔序列Key words
Lactic acid bacteria/clustered regularly interspaced short palindromic repeats (CRISPR)/homology/repeats/spacers分类
轻工纺织引用本文复制引用
李婉,张丹青,王娜娜,霍贵成..乳酸菌CRISPR-Cas系统同源性分析[J].食品工业科技,2016,37(11):134-140,146,8.基金项目
国家自然科学基金(31171717) （31171717）
国家“863计划”(2012AA022108). （2012AA022108）
