食品工业科技2016，Vol.37Issue(11)：165-168,173,5.DOI:10.13386/j.issn1002-0306.2016.11.026
麦芽三糖生成酶高产菌株的选育
Breeding of high-yielding strains of maltotriose-forming enzyme
摘要
Abstract
The Microbacterium imperiale of Mebl-012 was selected as the original strain,and the composite mutagenesis of Atmospheric and room temperature plasma(ARTP)-UV were used to treat it,in order to obtain a strain of high maltotriose-forming enzyme activity.The bacterial suspension under different ARTP mortality rates were mixed,and then were coated filter plate,followed by fermentation rescreening.Eventually a high-yielding strain Microbacterium imperiale of Metp-57 was screened out,production of this strain reached 241.32 U/mL,which was increased by 107.26％ comparing with the original strain Mebl-012(yield of 116.43 U/mL).Choosing high-producing strain Metp-57 as starting strain,UV mutagenesis was conducted,maltotriose-forming enzyme activity of the mutant Metu-24 reached 408.41 U/mL,which was increased by 250.77％ compared with the original strain Mebl-012,and its genetic stability was good.Compared with the original strain Mebl-012,the growth rate of metant strains Metu-24 was significantly improved.Hydrolyzing starch by this enzyme,maltotriose was predominant component,maltotriose content reached 46.7％ in maltotriose syrup.关键词
麦芽三糖生成酶/蛾微杆菌/常压室温等离子体诱变/紫外诱变Key words
maltotriose-forming enzyme/microbacterium imperiale/ARTP/UV mutagenesis分类
轻工纺织引用本文复制引用
朱明,乔长晟,李文军..麦芽三糖生成酶高产菌株的选育[J].食品工业科技,2016,37(11):165-168,173,5.基金项目
天津市科委科技支撑项目(14ZCZDTG00025)资助. （14ZCZDTG00025）
