食品工业科技2016，Vol.37Issue(11)：242-248,7.DOI:10.13386/j.issn1002-0306.2016.11.041
响应面法优化酶解鸢乌贼制备抗氧化肽的工艺研究
Optimization of enzymatic preparation of antioxidant peptides from purpleback flying squid (sthenoeuthis oualaniensis) protein by response surface methodology
摘要
Abstract
Response surface methods were used to optimize the hydrolysis conditions of purpleback flying squid (Sthenoeuthis oualaniensis) for production of antioxidant peptides.Papain was the optimum enzyme selected by the single-factor tests.Based on the single-factor tests,enzyme amount,liquid-material ratio and hydrolysis time were determined by single-factor tests as independent variables,1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity as the response value.The model of quadratic regression design was established.The hydrolysis conditions were adjusted to that enzyme amount was 0.66％ (w/w),liquid-material ratio was 10.2(v/w),hydrolysis time was 380 min.Under such conditions,1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was 89.80％.The result was consistent with the model prediction.In addition,amino acid analysis showed that compared with purpleback flying squid protein,the content of essential amino acids of enzymatic hydrolysis product was increased to 2.2 times,antioxidative amino acids such as methionine and tryptophan was increased to 7.2,25.6 times,respectively.关键词
鸢乌贼/酶解/响应面法/抗氧化肽/氨基酸Key words
purpleback flying squid/enzymatic hydrolysis/response surface methodology/antioxidant peptides/amino acid分类
轻工纺织引用本文复制引用
杨贤庆,吴静,胡晓,李来好,吴燕燕,林婉玲,黄卉,裘超颖,马海霞..响应面法优化酶解鸢乌贼制备抗氧化肽的工艺研究[J].食品工业科技,2016,37(11):242-248,7.基金项目
农业部财政重大专项(NFZX2013) （NFZX2013）
国家现代农业产业技术体系建设专项(CARS-49) （CARS-49）
“十二五”国家科技支撑计划项目(2012BAD28B06) （2012BAD28B06）
国家自然科学基金青年科学基金项目(31301454) （31301454）
广东省海洋渔业科技与产业发展专项(A201401C02) （A201401C02）
广东省科技计划项目(2013B021100013) （2013B021100013）
中央级公益性科研院所基本科研业务费专项资金项目(2012TS03). （2012TS03）
