食品工业科技2016，Vol.37Issue(11)：281-283,288,4.DOI:10.13386/j.issn1002-0306.2016.11.049
GC-MS法检测婴儿配方奶粉甘油三酯中Sn-2位棕榈酸
Determination of palmitic acid in the Sn-2 position in infant formulas by GC-MS
摘要
Abstract
To establish a highly efficient and accurate determination of Sn-2 palmitic acid in triglycerides of infant formulas.Lipids of milk powder were extracted by the method of ISO14156.The Sn-1,3 fatty acids in the triglycerides were specially hydrolyzed by pancreatic lipase.The 2-monoglyceride was separated and prepared by solid phase extraction (SPE),and analyzed by gas chromatography-mass spectrometry (GC-MS).Results showed that the content of palmitic acid esterified at the Sn-2 position was 40.5％,40.3％,40.8％.The average spike recoveries for palmitic acid were 91.7％～ 98.5％,and the relative standard deviation was 1.56％.The developed method was proved to be simple,precise,accurate and reliable,thereby being suitable for the determination of Sn-2 palmitic acid in infant formulas.关键词
婴儿配方奶粉/Sn-2位棕榈酸/氨基固相萃取柱(SPE)/气相色谱-质谱联用(GC-MS)Key words
infant formulas/Sn-2 palmitic acid/solid phase extraction (SPE)/gas chromatography-mass spectrometry (GC-MS)分类
轻工纺织引用本文复制引用
林曼,陈历俊,董学艳,闵伟红,姜铁民..GC-MS法检测婴儿配方奶粉甘油三酯中Sn-2位棕榈酸[J].食品工业科技,2016,37(11):281-283,288,4.基金项目
十二五国家科技支撑计划(2013BAD18B04) （2013BAD18B04）
北京市科技计划(D141100004814001). （D141100004814001）
