食品工业科技2016，Vol.37Issue(11)：299-304,6.DOI:10.13386/j.issn1002-0306.2016.11.053
四环素分子印迹电化学传感器的制备及快速检测牛奶和猪肉中四环素类药物残留
Preparation of tetracycline moleculary imprinting electrochemical sensor and rapid detection of tetracycline residues in milk and pork
摘要
Abstract
A novel molecular imprinted electrochemical sensor was prepared by electro-polymerization on thesurface of a glassy carbon electrode in phosphate buffer solution,and tetracycline was used as template moleculer,o-phenylenediamine(OPD) as functional monomer.The sensor could specifically recognize the template molecule and its analogues.In the experiment,the HAc-NaAc buffer solution containing 1 mol/L KCL and 5 mmol/L K3 [Fe(CN)6] was used as characterization solution,the electrochemical sensor response characteristics were researched,and the preparation and detection conditions of the sensor were optimized by differential pulse voltammetry(DPV)and cyclic voltammetry(CV).The results showed that the sensor had a good selectivity under the optimal conditions,the linear relationships between DPV peak current difference and the concentrations of tetracycline,oxytetracycline and doxycycline hyclate were found in the range of 0.002～0.02 mmol/L with the detection limits between 6.0 x 10-7 and 7.5 x 10-7 mol/L.The sensor in methanol-H2SO4 solution was renewable,and had good reproduction,stability and good selectivity to tetracycline and its analogues.The tetracycline,oxytetracycline and doxycycline in real samples,such as milk and pork,were detected by the sensor,the average recoveries were between 82.8％ and 97.4％,the relative standard deviations (RSD)were less than 4.43％.This sensor was simple,sensitive,low cost,and had a good stability,which provided it a good application prospect.关键词
四环素/分子印迹聚合物/电化学传感器/快速检测Key words
tetracycline/molecular imprinting polymer/electrochemical sensor/rapid detection分类
轻工纺织引用本文复制引用
高林,高文惠..四环素分子印迹电化学传感器的制备及快速检测牛奶和猪肉中四环素类药物残留[J].食品工业科技,2016,37(11):299-304,6.基金项目
河北省科技计划项目(16275503D) （16275503D）
河北省食品药品监督管理局食品药品安全科技计划项目(ZD2015015). （ZD2015015）
