食品工业科技2016，Vol.37Issue(11)：310-313,4.DOI:10.13386/j.issn1002-0306.2016.11.055
超高效液相色谱串联质谱法检测玛咖产品中非法添加西地那非
Detection of sildenafil illegally added in maca products by liquid chromatography tandem mass spectrometry
摘要
Abstract
The purpose was to establish a method for determination of sildenafil in Maca products by UPLC-MS/ MS.The samples were separated on Agilent SB-C18 column (1.8 iμm,2.1 mm x 50 mm)and eluted using mobile phase of methanol(10 mmol/L ammonium acetate):water(10 mmol/L ammonium acetate) (90∶ 10,v/v)at a flow rate of 0.3 mL/min,the injection volume was 5 μL.MS condition was positive ion mode,collecting multiple reaction monitoring(MRM) mode.Precursors to product ion transitions of m/z 475.3 ＞58.1 was to quantify sildenafil,and m/z 475.3 ＞282.9 was used as qualifying ion for sildenafil.The collision energy was 50 and 60 eV,respectively.The method was linear in the concerntration range of 0.2 ～ 20 iμg/L,and the correlation coefficient was 0.9994.The method was accurate,reliable and highly sensitive,and hence it can be applied in the determination of sildenafil in Maca products.关键词
玛咖/西地那非/UPLC-MS/MSKey words
Maca/Sildenafil/UPLC-MS/MS分类
轻工纺织引用本文复制引用
李绍辉,吴寒秋,许秀丽,章飞芳,冯峰,张峰,梁鑫淼..超高效液相色谱串联质谱法检测玛咖产品中非法添加西地那非[J].食品工业科技,2016,37(11):310-313,4.基金项目
国家重大科学仪器设备开发专项项目(2012YQ14000806) （2012YQ14000806）
质检公益性项目(201310134). （201310134）
