食品工业科技2016，Vol.37Issue(11)：332-337,6.DOI:10.13386/j.issn1002-0306.2016.11.060
反油酸处理对人HepG2细胞代谢组的影响
Effect of elaidic acid on the metabolomic changes of human HepG2 cells
摘要
Abstract
The human HepG2 cells were used to investigate the metabolomic responses of HepG2 cells to the exposure of elaidic acid,with the aim to reveal the molecular mechanisms underlying its harmful effects on human health.Based on the preliminary result of toxicological assay,HepG2 cells were treated with 1.2 mmol/L and 1.5 mmol/L elaidic acid for 6 and 12 h,and the metabolome changes of these cells were analyzed by using a non -targeted platform composing of UPLC-MS/MS and GC-MS.Statistic data revealed that the treatment of elaidic acid resulted in remarkable metabolome changes in HepG2 cells in a time-dependent way.Elaidic acid treatment accelerated the pathway of fatty acid synthesis,led to the significant changes in phospholipid metabolism,the reconstruction of cell membrane,and the increase of the levels of intermediates involved in cholesterol and acylglycerol metabolism.Eladic acid treatment also enhanced the sugar and amino acid pathways,which provided fuel for the accelerated synthesis of fatty acids.These in vitro metabolomic data provide novel insights into the detrimental effects of trans-fatty acids on human health.关键词
反油酸/反式脂肪酸/代谢组学/胆固醇代谢/HepG2细胞Key words
elaidic acid/trans-fatty acids/metabolomics/cholesterol metabolism/HepG2 cells分类
轻工纺织引用本文复制引用
刘莹,刘会昌,石建新..反油酸处理对人HepG2细胞代谢组的影响[J].食品工业科技,2016,37(11):332-337,6.基金项目
国家基础研究项目(2012CB72804). （2012CB72804）
