食品工业科技2016，Vol.37Issue(11)：338-341,4.DOI:10.13386/j.issn1002-0306.2016.11.061
蓝莓花色苷对过氧化氢所致血管内皮细胞凋亡的保护作用
Protective effects of blueberry anthocyanins on hydrogen peroxide-induced injury of vascular endothelial cells
摘要
Abstract
Objective:To investigate the protective effects of blueberry anthocyanins on hydrogen peroxide(H2O2)-induced apoptosis of vascular endothelial cells.Methods:The EVC-304 cells cultured in logarithmic growth phase were incubated with 100 iμmol/L H2O2 for another 12 h after pretreatment with blueberry anthocyanins(10,20,and 40 tμg· mL-1)for 24 h.Cell viability was determined by MTT assay,the change of cell apoptosis were measured with cytoanalyzer,and the expression levels of Cleaved Caspase-3,Cyto c,Bax,and Bcl-2 were detected by Western blotting.Results:Compared with the normal control group,the survival rate of H2O2-treated cells was decreased and the apoptosis ratio was increased significantly (p ＜ 0.01),the proportion of cleaved Caspase-3,Cytoc and Bax/Bcl-2 was increased (p ＜ 0.01).Compared with H2O2-treated group,the survival rate of cells in blueberry anthocyanins-pretreated groups was increased significantly (p ＜ 0.01),the apoptosis rate was decreased significantly(p ＜0.01).The expression of intracellular cleaved caspase-3,Cyto c and Bax/Bcl-2 was decreased (p ＜0.01).Conclusion:Blueberry anthocyanin had protective effect on hydrogen peroxide-induced vascular endothelial cells apoptosis and the possible mechanism may be associated with protection the function of mitochondrion.关键词
蓝莓花色苷/细胞凋亡/过氧化氢/线粒体损伤Key words
blueberry anthocyanins/apoptosis/hydrogen peroxide/mitochondrion injuries分类
轻工纺织引用本文复制引用
李亚巍,昌盛,梁承武,王黎明,金瑛..蓝莓花色苷对过氧化氢所致血管内皮细胞凋亡的保护作用[J].食品工业科技,2016,37(11):338-341,4.基金项目
国家自然科学基金(21102055)资助项目. （21102055）
