食品工业科技2016，Vol.37Issue(14)：78-82,89,6.DOI:10.13386/j.issn1002-0306.2016.14.007
玉米赤霉烯酮一步ELISA法的建立及应用
Development and application of one-step enzyme-linked immunosorbent assays for zearalenone
摘要
Abstract
With aim of developing a speed,accurate,one-step enzyme-linked immunosorbent assay (ELISA) for the detection of zearalenone in feed and feed stuffs was set.Hapten was synthesized firstly,conjugated then to carrier proteins for complete antigen,which was used as antigen in mice for monoclonal antibody.Concentrations of coating antigen and antibody were determined with chessboard format.A stable analytical system was established and the specificity of monoclonal antibody was evaluated.The results showed that the detection of limit of this developed ELISA for zearalenone in pig feed、cow feed、maize pulp and wheat bran was 96.2,92.7,80.5 and 86.7 μg/kg,respectively.Feed samples were spiked with zearalenone and the recoveries of ELISA were 79.4％ ～ 104.9％.There was no distinct difference between the results of WDWK and RIDA kits.This study could bring a technical basis for the monitor of zearalenone.关键词
玉米赤霉烯酮/半抗原/单克隆抗体/酶联免疫吸附分析法(ELISA)/饲料及其原料Key words
zearalenone/hapten/monoclonal antibody/enzyme-linked immunosorbent assays (ELISA)/feed and feed stuffs分类
轻工纺织引用本文复制引用
王文珺,刘怡菲,韩霄,王照鹏,苏丽芳,敖海阔,吴雨洋..玉米赤霉烯酮一步ELISA法的建立及应用[J].食品工业科技,2016,37(14):78-82,89,6.基金项目
河北省科技计划项目(15277107D). （15277107D）
