食品工业科技2016，Vol.37Issue(14)：191-195,5.DOI:10.13386/j.issn1002-0306.2016.14.030
大肠杆菌丝氨酸转运系统单基因敲除对丝氨酸生产的影响
Effect of single-gene knockout of L-serine transport system on L-serine production in Escherichia coli
摘要
Abstract
Transport system modification is an important method in strain improvement.It has been reported that the uptake of L-serine was regulated by four genes of sdaC,cycA,sstTand tdcC in Escherichia coli.ln this study,the four single-gene knockout mutant strains of sdaC,cycA,sstT and tdcC were constructed,respectively.The fermentation results showed that the sdaC and cycA knockout mutants produced 16.3 g/L and 14.1 g/L L-serine,respectively,which were 43％ and 25％ higher than that of the control strain.However,the production of sstT and tdcC mutants were both similar to the control strain.关键词
转运系统/L-丝氨酸/大肠杆菌/基因敲除Key words
transport system/L-serine/Escherichia coli/gene knockout分类
轻工纺织引用本文复制引用
崔云风,石斌超,李晶,赵志军,史吉平,张霞..大肠杆菌丝氨酸转运系统单基因敲除对丝氨酸生产的影响[J].食品工业科技,2016,37(14):191-195,5.基金项目
国家自然科学基金青年科学基金项目(31300048). （31300048）
