食品工业科技2016，Vol.37Issue(14)：250-256,7.DOI:10.13386/j.issn1002-0306.2016.14.042
柴达木枸杞类胡萝卜素提取工艺优化及抗氧化活性研究
Extraction optimization and antioxidant activity for carotenoid in Qaidam Chinese wolfberry
摘要
Abstract
The Qaidam Chinese Wolfberries were used as the raw material in carotenoid extracting.In order to improve extraction content,the method of ultrasonic-assisted organic solvent extraction was adopted.Based on the single factor experiments,solid-liquid ratio,the ratio of the extraction solvent and the extraction time were optimized by Box-Behnken Design.The inhibitory rate of DPPH scavenging activity was analyzed antioxidant activity of carotenoid.Under optimal extraction conditions,the contents of carotenoid in Qaidam Chinese Wolfberry from different producing areas and different picking period were determined.By the optimizing of response surface method,the optimalizing technique was obtained,which was that the extraction solvent was petroleum ether and ethanol mixtures(v/v =2.6∶1),the solid-liquid ratio was 1 ∶ 60 (g/mL),extraction time and extraction times were 41 min and 1,respectively.The content of carotenoid in Qaidam Chinese wolfberry was 321.52 mg/100 g under optimized conditions.The half maximal inhibiting concentration(IC50) of DPPH scavenging activity of carotenoid was 34.09 μg/mL.There were statistically significant differences in carotenoid content in Qaidam Chinese Wolfberry compared with different producing areas and different picking period (p ＜ 0.05).关键词
柴达木枸杞/类胡萝卜素/响应曲面/抗氧化活性Key words
Qaidam Chinese wolfberry/carotenoid/response surface methodology/antioxidant activity分类
轻工纺织引用本文复制引用
吴有锋,马世震,谭亮,冯海生,李彩霞..柴达木枸杞类胡萝卜素提取工艺优化及抗氧化活性研究[J].食品工业科技,2016,37(14):250-256,7.基金项目
中国科学院藏药现代化重点实验室项目(Y4496110Z1). （Y4496110Z1）
