食品工业科技2017，Vol.38Issue(5)：140-144,150,6.DOI:10.13386/j.issn1002-0306.2017.05.018
米邦塔仙人掌多糖的提取纯化及体外抗氧化活性
Extraction, purification and antioxidant activity of polysaccharides from Opuntia Milpa Alta in vitro
林款 1徐丛玥 1梁征 1茹琴 1李超英1
作者信息
- 1. 江汉大学武汉生物医学研究院,湖北武汉430056
- 折叠
摘要
Abstract
Objective:To investigate the purification of polysaccharides from Opuntia Milpa Alta and the antioxidant activity in vitro.Methods:Crude polysaccharides from Opuntia Milpa Alta (MAP)was extracted by water extraction and alcohol sedimentation.Two polysaccharides named MAP1 and MAP2 were achieved after MAP was purified through DEAE-cellulose-52,and another polysaccharide named MAP3 was achieved through DEAE-Sepharose-CL-6B.The relative molecular mass of polysaccharides was analyzed by gel permeation chromatography (GPC) and the antioxidant activity was investigated in vitro.Results:The relative molecular mass (Mn) of MAP1 was 476.9 ku,MAP2 contained three relative molecular mass (Mn) 5449,9724,529 ku,MAP3 contained 13270,15.87,474 ku.The results of antioxidant activity in vitro showed that,MAP had the highest total antioxidant capacity,the activity of MAP2 and MAP3 were higher than MAP in scavenging hydroxyl radical,superoxide anion and DPPH radical,and MAP2 had a higher clearance rate than MAP3 in the whole antioxidant indicators.Conclusion:The results indicated that MAP2 had a better antioxidant activity,while antioxidant activity of polysaccharide in vitro was associated with relative molecular mass.关键词
米邦塔仙人掌/多糖/纯化/相对分子质量/体外抗氧化Key words
Opuntia Milpa Alta/polysaccharides/purification/relative molecular mass/antioxidant activity in vitro分类
轻工纺织引用本文复制引用
林款,徐丛玥,梁征,茹琴,李超英..米邦塔仙人掌多糖的提取纯化及体外抗氧化活性[J].食品工业科技,2017,38(5):140-144,150,6.
