食品工业科技2017，Vol.38Issue(5)：145-150,6.DOI:10.13386/j.issn1002-0306.2017.05.019
半纤维素降解菌J-25的筛选、鉴定及产酶特性研究
Screening, identification and enzymatic analysis of hemicellulose-degrading strain J-25
摘要
Abstract
A hemicellulose degrading bacteria was isolated with rotting wood as a source.Hydrolysis spot diameter measurement of hemicellulose plate and extracellular enzyme activity was used.The strain J-25 was identified as Cellulomonas by 16S rDNA sequencing analysis,named Cellulomonas sp.J-25.And characteristics of enzyme production and enzymatic properties were studied.The results indicated that fermentation conditions for enzyme production of strain J-25 was:temperature 30 ℃,the initial pH of 7.0,bran concentration of 20 g/L,yeast powder concentration of 10 g/L,liquid volume of 50 mL(250 mL flask),hemicellulose activity may reach 62.8 U/mLThe optimum enzyme reaction conditions were as follows:pH6.5,temperature of 40 ℃,substrate concentration of 15 g/mL,the reaction time of 30 min.These will provide experimental evidence for hemicellulose biodegradation.关键词
半纤维素/降解菌/产酶条件/酶学性质/16S rDNAKey words
hemicellulose/degrading-bacteria/fermentation conditions/enzymatic properties/16S rDNA分类
轻工纺织引用本文复制引用
姜立春,王成红,刘羽,林寿露,赵丽萍,阮期平..半纤维素降解菌J-25的筛选、鉴定及产酶特性研究[J].食品工业科技,2017,38(5):145-150,6.基金项目
四川省科技厅项目(2016JY0038) （2016JY0038）
四川省大学生创新创业训练项目(201510639010,201510639066). （201510639010,201510639066）
