食品工业科技2017，Vol.38Issue(5)：163-167,5.DOI:10.13386/j.issn1002-0306.2017.05.022
芒果露水斑病病原菌分离鉴定及其拮抗菌筛选
Isolation and identification of pathogen causing mango dew blotch disease and screening of its antagonistic microorganisms
摘要
Abstract
Objective:The project aims to clarify the pathogen of the mango dew blotch disease,screen its antagonistic microorganisms in order to lay theoretical basis for the further prevention of the disease.Methods:Scanning electron microscope was used to observe the condition of the peel of diseased fruit.Conventional tissue separation method was applied to separate disease tissue.And the antagonistic microorganisms were screened by the flat-stand method.The pathogen and antagonistic microorganisms were identified in morphological and molecular biological methods.Results:It was found that mango dew blotch disease was caused by fungus.And the pathogen was identified as Cladosporium cladosporioides.Eight antagonistic strains against Cladosporium cladosporioides were isolated and screened from the soil in mango garden.The strain JS-8 and Y-1 showed the highest and most stable antagonistic effect (p ＜ 0.05).Results showed that the inhibition zone diameters were 15.17 mm and 14.93 mm.Based on morphological,physiological and biochemical characteristics,and molecular biological methods,JS-8 and Y-1 were identified as Burkholderia gladioli,Debaryomyces hansenii.Conclusions:Mango dew blotch disease was mainly caused by Cladosporium cladosporioides.Burkholderia gladioli and Debaryomyces hansenii can be used for potential biocontrol strains of Mango dew blotch disease.关键词
芒果露水斑病/病原菌/拮抗菌/鉴定/筛选Key words
mango dew blotch disease/pathogen/antagonistic microorganisms/identification/screening分类
轻工纺织引用本文复制引用
井敏敏,田亚琴,王润菡,邵远志..芒果露水斑病病原菌分离鉴定及其拮抗菌筛选[J].食品工业科技,2017,38(5):163-167,5.基金项目
农业部南亚热作项目(14RZNJ-59) （14RZNJ-59）
海南省重点研发计划项目(ZDYF2016043). （ZDYF2016043）
