食品工业科技2017，Vol.38Issue(5)：246-251,257,7.DOI:10.13386/j.issn1002-0306.2017.05.038
大孔树脂分离纯化菠萝蜜果皮黄酮工艺及其抗氧化活性研究
Study on antioxidant activity of flavonoids extracted from jackfruit skin separated and purified by macroporous resin technology
摘要
Abstract
Flavonoid crude extracts distilled from jackfruit skin were selected as test solution in this study.NKA resin was picked up as the best separation and purification resin from six kinds of macroporous resins with different polarity through static adsorption analysis,meanwhile,the analytical conditions of dynamic adsorption of NKA column chromatography were explored as well.The results showed the optimized parameters of analytical conditions were sample concentration of 0.90 mg/mL,the sample volume of 200 mL,and the elution dose of 120 mL.The best purification process was obtained by the single factor and orthogonal experiments which were 4 mL/min,pH5 and 70％ ethanol concentration on sampling and elution flow rate,sampling pH value and ethanol concentration.The recovery rate of flavonoids was as high as 96.02％ in this cases.The purity of flavonoids was increased from 16.74％ to 84.42％ and increased about 4.04 times after post-column,which indicated that NKA resin had a significant effect on the enrichment and purification of flavonoids from jackfruit skin.Results of in vitro antioxidant activity showed that the purified flavone with NKA column chromatography exhibited significantly enhancement with increased purity of flavonoids compared with that unpurified.关键词
菠萝蜜果皮/黄酮/分离纯化/体外抗氧化活性Key words
jackfruit peel/separation and purification/antioxidant activity in vitro分类
轻工纺织引用本文复制引用
邓梦琴,林晓瑛,张明,宋贤良,黄苇..大孔树脂分离纯化菠萝蜜果皮黄酮工艺及其抗氧化活性研究[J].食品工业科技,2017,38(5):246-251,257,7.基金项目
公益性行业(农业)科研专项(201303077). （农业）
