食品工业科技2017，Vol.38Issue(5)：361-365,5.DOI:10.13386/j.issn1002-0306.2017.05.060
头孢克肟诱导肠道菌群紊乱动物模型的建立
Establishment of mice model for intestinal dysbacteria induced by cefixime dispersible tablets
摘要
Abstract
Objective:To produce the mouse models of intestinal dysbacteria which induced by cefixime dispersible tablets.Methods:female SPF grade BALB/c 36,weight 18 ～22 g,intestinal dysbiosis mice treated with cefixime dispersible tablets,low dose (187.75 mg/(kg· d)),medium dose (375.5 mg/(kg· d)) and high dose (563.25 mg/(kg· d)).Control mice received sterile deionized water by oral gavage.On the 8th day,the mice were euthanized by cervical dislocation.The caecum contents were collected in a sterile 2 mL centrifuge tube.Compare the shift in the intestinal microbial by vital cell counting and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis.Result:The Enterococcus and E.coli were promoted and Lactobacillus was suppressed.The reduction of diversity and abundance was shown in middle and higher doses group.Conclusion:The model of intestinal dysbacteria builded successfully.关键词
头孢克肟/肠道菌群/聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)Key words
cefixime dispersible tablets/intestinal microflora/polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)分类
轻工纺织引用本文复制引用
李静,吕晓玲,吕冬雪,王梦姝,赵胜男,赵焕焦..头孢克肟诱导肠道菌群紊乱动物模型的建立[J].食品工业科技,2017,38(5):361-365,5.基金项目
国家科技支撑计划项目(2011BAD23B02). （2011BAD23B02）
