食品工业科技2017，Vol.38Issue(6)：54-61,78,9.DOI:10.13386/j.issn1002-0306.2017.06.002
HS-SPME结合GC-O-MS技术分析不同大曲中的香气活性化合物
Identification of aroma-active compounds in Daqu by HS-SPME-GC-MS/O
摘要
Abstract
The aroma compounds of Daqu were analyzed by headspace-solid-phase microextraction(HS-SPME) combined with gas chromatography-olfactometry-mass spectrometry(GC-O-MS).At the same time,the conditions of fiber type,adsorption time,extraction temperature and sample amount for analysis of volatile compounds in Daqu samples were optimized.The conditions of SPME were optimized by single factor experiment,such as fiber type,adsorption time,extraction temperature and sample amount.The optimum extraction conditions were 1.5 g sample,50/30 μm DVB/CAR/PDMS fiber,extraction time 30 rain and extraction temperature 50 ℃.With the potimal conditons,69 volatile compounds were identified from the tested Daqu sample,including 9 alcohols,13 esters,8 aldehydes,4 ketones,3 acids,9 pyrazines,8 aromatic compounds,5 phenols,2 heterocyclic,3 alkanes and 5 others.17 aroma-active compounds were detected by GC-O-MS,14 compounds were identified.The key aroma-active compounds were 2-ethyl-3,5-dimethyl pyrazine and phenylacetaldehyde.Their odor activity values (OAVs)and their aroma intensities were stronger than others.They contribute honey-like,baked potato-like,and the all aroma-active compounds constitute the unique aroma of Daqu.关键词
大曲/顶空-固相微萃取/气相色谱-嗅闻-质谱/风味活性化合物/香气活性值Key words
Daqu/headspace solid-phase microextraction (HS-SPME)/gas chromatography-olfactometry-mass spectrometry (GC-O-MS)/aroma-active compounds/odor activity value分类
轻工纺织引用本文复制引用
孟维一,黄明泉,孙宝国,郑福平,孙金沅,孙啸涛,李贺贺..HS-SPME结合GC-O-MS技术分析不同大曲中的香气活性化合物[J].食品工业科技,2017,38(6):54-61,78,9.基金项目
国家自然科学基金(31471665) （31471665）
北京市属高等学校高层次人才引进与培养计划项目(CIT&tcd201404034). （CIT&tcd201404034）
