食品工业科技2017，Vol.38Issue(6)：101-105,109,6.DOI:10.13386/j.issn1002-0306.2017.06.010
海洋胶原低聚肽中抗氧化肽的分离及鉴定
Separation and identification of antioxidant peptides derived from marine collagen oligopeptides
摘要
Abstract
In order to investigate the activity and structure of antioxidant peptides derived from marine collagen oligopeptides (MCOP),MCOP was prepared by the method of two-step enzymolysis.On the basis of analysis of molecular weight distribution,the antioxidant activity of MCOP was determined using the method of DPPH radical scavenging activity as the indicator.The results showed that the major molecular weight of MCOP was less than 1000 u(90.79％),and small peptides with a molecular weight range of 132～576 u were dominated.The half-inhibition concentration (IC5o) of DPPH radical scavenging activity was approximately 8.0 mg/mL.Then,MCOP was separated and purified by reversed phase high performance liquid chromatography(RP-HPLC)and the DPPH radical scavenging activity of 11 fractions collected was assayed.The results indicated that all the fractions had the effect higher than MCOP.Finally,the identification of peptides in the 11 fractions was performed on the Q-TOF mass spectrometer and the DPPH radical scavenging activity of the 15 identified peptides was investigated.The results indicated that all the peptides exhibited DPPH radical scavenging activity and Gly-Arg (GR),ArgGlu-Arg(RER) and Ala-Pro(AP)showed stronger activities than MCOP.GR,RER and AP were potent antioxidant peptides.This study provides certain theoretical basis for the development and utilization of MCOP.关键词
海洋胶原低聚肽/抗氧化肽/分子量分布/分离纯化/结构鉴定Key words
marine collagen oligopeptides/antioxidant peptides/molecular weight distribution/separation and purification/structural identification分类
轻工纺织引用本文复制引用
刘文颖,徐姗姗,谷瑞增,鲁军,潘兴昌,蔡木易..海洋胶原低聚肽中抗氧化肽的分离及鉴定[J].食品工业科技,2017,38(6):101-105,109,6.基金项目
十三五国家重点研发计划(2016YFD0400604) （2016YFD0400604）
国家高技术研究发展计划(863计划)项目(2013AA102205-02). （863计划）
