食品工业科技2017，Vol.38Issue(6)：142-146,5.DOI:10.13386/j.issn1002-0306.2017.06.019
复合酶法高效提取海带中褐藻糖胶
Efficient extraction of fucoidan from Laminaria japonic by compound enzymes
摘要
Abstract
This study was designed to extract the fucoidan from Laminaria japonicaby compound enzyme.By optimizing the extraction conditions and comparing with traditional extraction method and other enzyme extraction method,our target was to develope an efficient method which might product more fucoidan.According to single factor experiment,the ratio of compound enzyme of cellulase,pectinase,xylanase,α-amylase and acid protease was 25∶ 10∶ 10∶1 ∶ 5.By using orthogonal test which was based onsingle factor experiment and the variance analysis,the optimal hydrolysis conditions for fucoidan extraction from Laminariajaponicawere 4.6％ compound enzyme,extraction temperature of 59 ℃,pH4.5,extraction time of 18 h and solidliquid ratio 1∶ 30.Under these conditions,the extraction yield of fucoidan was 2.04％ and an extraction rate was 90.20％.It was significantly higher than the existing research reports.This study established a new efficient extraction of fucoidan by compound enzymes,to provide theoretical and practical foundation for further scale-up.关键词
褐藻糖胶/复合酶/海带/多糖Key words
Laminaria japonica/compound enzyme/fucoidan/polysaccharide分类
轻工纺织引用本文复制引用
杨晓雪,董学前,张永刚,王伟,杜金华,刘建军..复合酶法高效提取海带中褐藻糖胶[J].食品工业科技,2017,38(6):142-146,5.基金项目
山东省科技重大专项支持项目(2015ZDZX05001). （2015ZDZX05001）
