食品工业科技2017，Vol.38Issue(6)：147-152,157,7.DOI:10.13386/j.issn1002-0306.2017.06.020
植物乳杆菌163产抗菌物质的发酵条件优化
Optimization of fermentation conditions of the production of antibacterial substances by Lactobacillus plantarum 163
摘要
Abstract
Optimization of fermentation conditions of the production of plantaricin by Lactobacillus plantarum 163,which was isolated from Chinese traditional pickled cabbage in our laboratory,was carried out using single-factor experiments,a Plackett Burman design(PB),a Box-Behnken design (BBD)and response surface methodology (RSM).The best concentration of the optimized medium was 55 g/L maltose syrup,20 g/L corn steep powder,8 g/L CH3COONa,6 g/L diammonium citrate,1.26 g/L Tween-80,and initial pH value 4.7,inoculum 2％.The inhibitory diameter has increased to 31.90 mm,which is 1.10 times lager than cultured in MRS medium.The optimization had not only improved the antimicrobial activity of the fermentation broth,but also found a food grade and cheap medium which could be used in large scale.关键词
植物乳杆菌163/响应面方法/植物乳杆菌素Key words
Lactobacillus plantarum 163/response surface methodology/plantaricin分类
轻工纺织引用本文复制引用
曾维伟,何镇宏,陆兆新,吕凤霞,别小妹,赵海珍,张充..植物乳杆菌163产抗菌物质的发酵条件优化[J].食品工业科技,2017,38(6):147-152,157,7.基金项目
国家科技支撑计划(2015BAD16B04). （2015BAD16B04）
