食品工业科技2017，Vol.38Issue(6)：185-188,194,5.DOI:10.13386/j.issn1002-0306.2017.06.027
蛋白核小球藻不同生长阶段油脂和脂肪酸组成的分析
Analysis of lipid content and fatty acid composition of Chlorella pyrenoidosain in different cultivation stages
摘要
Abstract
Objective:In order to increase more deeply understanding the lipid synthesis of C.pyrenoidosa,the changes of the lipid content and the fatty acid composition with the cultivation stage were observed.Methods:The algal biomass was collected at each cultivation stage by centrifugation of cultivation broth,the lipid was extracted and the fatty acid compositions were analysed.Results:The results showed that the total content of lipid was 21.18％ in the nitrogen deficiency stage,which was 1.54 and 1.32 fold of that at logarithmic growth phase and stationary phase,respectively.The GC results indicated that long chain fatty acids of C 16 ～ C1 8 were the dominant fatty acids of C.pyrenoidosa,and most of them were oleic acid (C18∶1) and linoleic acid(C18∶ 2),accounting for more than 35.90％ of the total fatty acids.Conclusion:Increasing total lipid content and polyunsaturated fatty acid accumulated in nitrogen deficiency stress stage,improving the lipid quality of C.pyrenoidosa.关键词
蛋白核小球藻/总脂含量/脂肪酸成分/生长期Key words
Chlorella pyrenoidosa/lipid content/fatty acid composition/cultivation stages分类
轻工纺织引用本文复制引用
杨慧敏,秦焰祯,杨青峰,朱宏波,胡雪琼,钟敏,李雁群..蛋白核小球藻不同生长阶段油脂和脂肪酸组成的分析[J].食品工业科技,2017,38(6):185-188,194,5.基金项目
广东省自然科学基金项目(2015A030313616) （2015A030313616）
湛江市科技计划项目(2015A03026). （2015A03026）
