食品工业科技2017，Vol.38Issue(6)：223-228,6.DOI:10.13386/j.issn1002-0306.2017.06.034
响应面法优化新疆奶花芸豆α-淀粉酶抑制剂的提取工艺
Optimization of extraction of alpha-amylase inhibitor from Phaseolus vulgaris by response surface methodology
于雪慧 1田童童 1张建1
作者信息
- 1. 石河子大学食品学院,新疆石河子832003
- 折叠
摘要
Abstract
Response surface methodology was applied to optimize the extraction conditions of alpha-amylase inhibitor from Phaseolus vulgaris.Based on the results of the single factor experiments,the effects of four independent variables (volume of extraction solvent,extraction pH value,extraction time and extraction temperature)on the o-AI inhibition rate were investigated by Box-Behnken experimental design of four factors and three levels.A quadratic polynomial regression equation of the forecasting model was set up.And the optimal extraction parameters to obtain the highest inhibition rate were as follows:the solvent volume was 200 mL,pH3.5,43 ℃ and 2.3 h for extraction per time.The inhibition rate under the optimum conditions was found to be 85.30％,which agreed with the predicted value of 87.82％.Results showed that the optimized method was stable and reliable.It could lay the foundation for the research,development of the Phaseolus vulgaris.关键词
奶花芸豆/α-淀粉酶抑制剂/响应面分析Key words
Phaseolus vulgaris/α-amylase inhibitor/response surface method分类
轻工纺织引用本文复制引用
于雪慧,田童童,张建..响应面法优化新疆奶花芸豆α-淀粉酶抑制剂的提取工艺[J].食品工业科技,2017,38(6):223-228,6.
