食品科学2017，Vol.38Issue(5)：7-13,7.DOI:10.7506/spkx1002-6630-201705002
牛血清白蛋白与槲皮素及花青素相互作用方式及其纳米颗粒特征的比较
Interaction Modes and Nanoparticle Characteristics of Bovine Serum Albumin with Quercetin and Anthocyanin
摘要
Abstract
The interaction modes and nanoparticle characteristics of bovine serum albumin (BSA) with quercetin (QUE) and anthocyanin (ACN) were comparatively investigated.The fluorescence of BSA was greatly quenched by QUE in both static (at low concentration) and dynamic (at high concentration) modes,while fluorescence quenching of BSA by ACN was just in a static mode.The binding constant of BSA with QUE was higher than that with ACN.BSA interacted with QUE and ACN by hydrophobic force and electrostatic force,respectively,to form BSA-QUE and BSA-ACN nanoparticles.The average diameters of BSA-QUE and BSA-ACN were 42.5 nm and 53.7 nm,respectively.The ξ-potentials of BSA-QUE and BSA-ACN were-25.64 and-21.50 mV,respectively.One mole of BSA could combine 8 moles of QUE or 10 moles of ACN.BSA-QUE nanoparticles were smaller and more stable than BSA-ACN nanoparticles.The DPPH and ABTS radical scavenging rates of BSA-QUE were higher than those of BSA-ACN.关键词
牛血清白蛋白/槲皮素/花青素/相互作用/纳米颗粒Key words
bovine serum albumin (BSA)/quercetin (QUE)/anthocyanin (ACN)/interaction/nanoparticle分类
轻工纺织引用本文复制引用
刘建垒,邢效娟,周瑞,景浩..牛血清白蛋白与槲皮素及花青素相互作用方式及其纳米颗粒特征的比较[J].食品科学,2017,38(5):7-13,7.基金项目
国家自然科学基金面上项目(31171676) （31171676）
