食品科学2017，Vol.38Issue(5)：14-20,7.DOI:10.7506/spkx1002-6630-201705003
梨幼果多酚的纯化及其抗氧化性
Purification and Antioxidant Activity of Polyphenols from Young Pear Fruits
摘要
Abstract
The polyphenols from young pear fruits were purified by 5 kinds of macroporous adsorption resins,and their antioxidant activity was determined.The results indicated that NKA-9 resin had better adsorption and desorption properties than four other resins.Therefore,it was the most suitable resin to purify polyphenols from young pear fruits.The optimum conditions for purification were as follows:after pH adjustment to 5.0,the polyphenol at 2 mg/mL was passed through the resin at a flow rate of 1 mL/min,followed by desorption with 70％ ethanol at a flow rate of 1 mL/min.Under these conditions,the purity of the polyphenols from young pear fruits increased from 6.23％ to 30.68％.Both the crude and purified samples had the ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH),2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals and nitrite,with IC50values of 151.07,152.48 and 490.35 μg/mL versus 122.12,130.78 and 392.09 μg/mL,respectively.Conclusively,the antioxidant capacity of the purified polyphenols was better than that of the crude sample.关键词
大孔树脂/梨幼果多酚/纯化/抗氧化性Key words
macroporous resins/polyphenols from young pear fruits/purification/antioxidation分类
农业科技引用本文复制引用
朱洁,王红宝,孔佳君,宋小飞,陶书田..梨幼果多酚的纯化及其抗氧化性[J].食品科学,2017,38(5):14-20,7.基金项目
国家自然科学基金面上项目(31372044) （31372044）
国家自然科学基金青年科学基金项目(31000888) （31000888）
国家高技术发展研究计划(863计划)项目(2011AA10020602) （863计划）
