食品科学2017，Vol.38Issue(5)：54-59,6.DOI:10.7506/spkx1002-6630-201705009
EGCG乙酰化分子修饰取代度影响因素分析
Major Factors Affecting Substitution Degree of Acetylated EGCG
摘要
Abstract
Acetylated epigallocatechin gallate (EGCG) was chemically synthesized in ethyl acetate solvent system for improving the lipid solubility and bioavailability of EGCG.The effects of acetyl anhydride dosage,catalyst pyridine dosage,ethyl acetate dosage,reaction temperature and reaction time on the substitution degree of acetylated EGCG were studied.As a result,it was found that 0.04 mL of acetic anhydride,0.02 mL of pyridine,20--60 mL of ethyl acetate,a reaction temperature of 20-25 ℃ and a reaction time of 1-3 h were optimal for the synthesis of acetylated EGCG with a degree of substitution in the range from 1 to 3 from 0.19 g of EGCG.The optimal synthesis conditions for EGCG acetylation that provided a substitution degree between 4 and 6 were 0.12-0.20 mL of acetic anhydride,0.06-0.20 mL of pyridine,10-20 mL of ethyl acetate,a reaction temperature of 17-25 ℃,and a reaction duration of 5-9 h,while those for a substitution degree of 7-8 were 0.40-0.80 mL of acetic anhydride,0.01-0.20 mL of pyridine,5-10 mL of ethyl acetate,a reaction temperature of 25-40 ℃,and a reaction duration of 5-9 h.关键词
表没食子儿茶素没食子酸酯/乙酰化/取代度/分子修饰/生物利用度Key words
epigallocatechin gallate (EGCG)/acetylation/substitution degree/molecular modification/bioavailability分类
轻工纺织引用本文复制引用
张建勇,江和源,崔宏春,王伟伟,施莉婷..EGCG乙酰化分子修饰取代度影响因素分析[J].食品科学,2017,38(5):54-59,6.基金项目
国家自然科学基金面上项目(31670692) （31670692）
中国农科院科技创新工程项目(CAAS-ASTIP-2016-TRICAAS) （CAAS-ASTIP-2016-TRICAAS）
