食品科学2017，Vol.38Issue(5)：233-238,6.DOI:10.7506/spkx1002-6630-201705038
多糖提取物与叶黄素联合干预对可见光诱导视网膜损伤的抗氧化作用
Protective Effect of Plant Polysaccharide Extracts Combined with Lutein on Visible Light-Induced Retinal Damage via an Antioxidant Mechanism
摘要
Abstract
The purpose of this study was to explore the protective effect of wolfberry and mulberry polysaccharides combined with lutein on visible light-induced retinal degeneration through their antioxidant activity.Pigmented rabbits were exposed to visible light to induce retinal light damage and then treated with different doses of polysaccharides extracted from a mixture of both dried fruits (1∶1,m/m) and lutein for 21 days.Afterwards,lipid peroxidation,protein oxidation,DNA oxidation biomarkers and antioxidant parameters in the retina were evaluated.The results revealed that light exposure induced significant increases in oxidative damage to lipids,proteins and DNA in the retina of pigmented rabbits.In contrast,co-treatment with medium and high doses of polysaccharide extracts and lutein decreased the contents of malondialdehyde (39.02％;54.08％,P ＜ 0.05),4-hydroxy azelaic acid (32.86％;41.78％,P ＜ 0.05),3-nitro-tyrosine (17.20％;19.69％) and 8-hydroxy-deoxyguanosine (6.12％;28.81％),paralleling with increased activities of catalase (1.96-fold;2.40-fold,P ＜ 0.05) and total antioxidant capacity (1.98-fold;2.11-fold,P ＜ 0.05).Accordingly,combined intervention with woltberry and mulberry polysaccharides and lutein can significantly alleviate visible light-induced retinal oxidative damage in pigmented rabbits.关键词
枸杞子多糖提取物/桑椹多糖提取物/叶黄素/可见光诱导视网膜损伤/抗氧化Key words
wolfberry polysaccharides/mulberry polysaccharides/lutein/visible light-induced retinal degeneration/antioxidant effect分类
医药卫生引用本文复制引用
邓乾春,臧茜茜,陈鹏,孟露曦,黄庆德,黄凤洪..多糖提取物与叶黄素联合干预对可见光诱导视网膜损伤的抗氧化作用[J].食品科学,2017,38(5):233-238,6.基金项目
公益性行业(农业)科研专项(201303072) （农业）
中国农业科学院油料作物研究所所长基金项目(1610172014006) （1610172014006）
