食品科学2017，Vol.38Issue(6)：1-5,5.DOI:10.7506/spkx1002-6630-201706001
瘤胃中干酪乳杆菌的亚油酸异构酶基因的克隆与表达
Cloning and Expression of the Linoleic Acid Isomerase Gene of Lactobacillus casei Isolated from Cattle Rumen
摘要
Abstract
Lactobacillus casei Fx was isolated from cattle rumen for its ability to transform linoleic acid into c9,t11-conjugated linoleic acid (CLA).Genomic DNA was extracted and a 1 700-bp linoleic acid isomerase (LAI) gene fragment was amplified from the strain by PCR.The gene fragment was purified and cloned into the plasmid pUCm-T-LAI to obtain recombinant plasmid pUCm-T-LAI by TA cloning.The recombinant plasmid and the expression plasmid pET-DsbA were subjected to double enzyme digestion and ligated to the recombinant expression vector pET-DsbA-LAL After identification by PCR and enzymatic digestion,the recombinant expression vector was transformed into E.coli BL21.The recombinant strain,having LAI activity,possessed the ability to specifically transform linoleic acid into c9,t11-CLA isomer.The results showed that the LAI gene from L.casei Fx was successfully cloned.This work makes it possible to understand the difference among the LAI genes in different rumen bacteria.关键词
共轭亚油酸/亚油酸异构酶/干酪乳杆菌/克隆/表达Key words
conjugated linoleic acid/linoleic acid isomerase/Lactobacillus casei/cloning/expression分类
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刘晓华,李慧美,柯颖笑,张凯强,李响敏,李欣,付金衡,李海星..瘤胃中干酪乳杆菌的亚油酸异构酶基因的克隆与表达[J].食品科学,2017,38(6):1-5,5.基金项目
国家自然科学基金地区科学基金项目(31260373) （31260373）
江西省重点研发计划项目(20161BBF60094) （20161BBF60094）
