食品科学2017，Vol.38Issue(6)：6-12,7.DOI:10.7506/spkx1002-6630-201706002
广谱抗菌芽孢杆菌的筛选及其抗酵母物质的纯化和鉴定
Screening of A Broad-Spectrum Antibacterial Bacillus and Purification and Identification of the Anti-Yeast Substances Produced by It
摘要
Abstract
One strain of Bacillus amyloliquefacien named LZ-5 was isolated from two wild honey samples collected from different regions.It was found to possess a broad spectrum of antimicrobial activity.Its secondary metabolites had strong antimicrobial activities against some kinds of food spoilage microorganisms,including Bacillus pumilus,Staphyloccocus aureus,Saccharomyces cerevisiae and Aspergillus oryzae.According to its morphological and cultural characteristics,physiological and biochemical properties and 16S rDNA sequence analysis,the strain was identified as Bacillus amyloliquefacien.The main anti-yeast substances produced by it were separated by organic solvent extraction,SephadexLH-20 colunm chromatography and high performance liquid chromatography.Liquid chromatography-electrospray ionization mass spectrometry was adopted to determine the molecular masses of the substances by comparison with standard compounds.The anti-yeast substances were preliminarily identified as a mixture of iturinA2,iturinA3 and iturinA6.关键词
解淀粉芽孢杆菌/筛选/抗菌物质/分离鉴定/iturinAKey words
Bacillus amyloliquefaciens/screening/antibacterial substance/separation and identification/iturinA分类
轻工纺织引用本文复制引用
石举然,别小妹,吕凤霞,陆兆新..广谱抗菌芽孢杆菌的筛选及其抗酵母物质的纯化和鉴定[J].食品科学,2017,38(6):6-12,7.基金项目
国家自然科学基金面上项目(31271936) （31271936）
