食品科学2017，Vol.38Issue(6)：13-19,7.DOI:10.7506/spkx1002-6630-201706003
香蕉MaWRKY31转录因子的特性及对MaACS1、MaACO1和MaSAG1的调控
Molecular Characterization of a Transcriptional Factor, MaWRKY31, and Its Regulation on MaACS1, MaACO1 and MaSAG1
摘要
Abstract
WRKY proteins are a class of plant-specific transcriptional factors (TFs),which play important roles in plant growth and development,leaf senescence and hormonal signaling.However,their relationship with banana fruit ripening and senescence remains unknown.In the present work,a WRKY TF,termed MaWRKY31,was obtained from banana fruit.Analysis of deduced amino acid sequence and phylogenetic tree revealed that MaWRKY31 contained a typical WRKY domain showing high homology with Arabidopsis thaliana WRKY31,and belonged to the Ⅱ b sub-group.Realtime quantitative polymerase chain reaction displayed that MaWRKY31 was induced by ethylene,and up-regulated following banana fruit ripening and senescence.Sub-cellular localization and transcriptional activity analysis revealed that MaWRKY31 was a nuclear protein with transcriptional activation activity.In addition,dual-luciferase transient expression assay demonstrated that MaWRKY31 could activate the promoter activities of the ethylene biosynthetic genes MaACS1 and MaACO1 and the ripening-related gene MaSAG1.Taken together,these results suggest that MaWRKY31 may be involved in banana fruit ripening and senescence,possibly via the regulation of the expression of genes related to ethylene biosynthesis and ripening,which deepens the understanding of the transcriptional regulation mechanism of banana fruit ripening and senescence.关键词
香蕉/果实成熟/WRKY转录因子/转录调控Key words
banana/fruit ripening and senescence/WRKY transcription factors/transcriptional regulation分类
轻工纺织引用本文复制引用
范中奇,卓远芳,符亚莉,谭小丽,邝健飞,陆旺金,陈建业..香蕉MaWRKY31转录因子的特性及对MaACS1、MaACO1和MaSAG1的调控[J].食品科学,2017,38(6):13-19,7.基金项目
国家自然科学基金面上项目(31372111) （31372111）
国家香蕉现代农业产业技术体系项目(CARS-32-09) （CARS-32-09）
