食品科学2017，Vol.38Issue(6)：20-26,7.DOI:10.7506/spkx1002-6630-201706004
植物乳杆菌FS5-5在盐胁迫下的转录组学分析
Transcriptomic Analyses of Lactobacillus plantarum FS5-5 against Salt Stress
摘要
Abstract
This investigation studied the expression of salt stress-related genes in Lactobacillus plantarum FS5-5,a salt-tolerance strain isolated from naturally fermented miso in northeastern China,at the level of transcription.Results showed that in the logarithmic growth phase,29 genes were significantly down-regulated,including four ones involved in carbohydrate transport and metabolism,nine ones involved in amino acids transport and metabolism,three ones involved in vitamin metabolism,six ones involved in nucleotide metabolism and seven ones involved in genetic information translation,ribosomal structure and biogenesis.Four genes involved in carbohydrate transport and metabolism were significantly upregulated,which might be closely associated with salt stress resistance of L.plantarum FS5-5.Three genes involved in vitamin metabolism and one gene involved in nucleotide metabolism were selected to be analyzed by real-time fluorescent quantitative polymerase chain reaction and the results showed that the expression of these genes exhibited the same trend in these two methods.The comprehensive analysis of the mechanism of action of L.plantarum FS5-5 to resist salt stress presented in this study which would provide a theoretical basis to improve the tolerance of strains in industrial production.关键词
植物乳杆菌/盐胁迫/实时定量聚合酶链式反应/转录组学Key words
Lactobacillus plantarum/salt stress/real-time fluorescent quantitative polymerase chain reaction/transcriptomics分类
生物科学引用本文复制引用
宋雪飞,郭晶晶,姜静,唐筱扬,张颖,乌日娜..植物乳杆菌FS5-5在盐胁迫下的转录组学分析[J].食品科学,2017,38(6):20-26,7.基金项目
国家自然科学基金青年科学基金项目(31000805) （31000805）
国家自然科学基金面上项目(31471713) （31471713）
中国博士后科学基金项目(2014M560395) （2014M560395）
辽宁省农业领域青年科技创新人才培养计划项目(2014048) （2014048）
辽宁省高等学校优秀人才支持计划项目(LR2015059) （LR2015059）
江苏省博士后科研资助计划项目(1402071C) （1402071C）
