食品科学2017，Vol.38Issue(6)：68-73,6.DOI:10.7506/spkx1002-6630-201706011
德氏乳杆菌保加利亚亚种ND02活的非可培养态诱导和复苏
Induction and Resuscitation of Viable but Non-culturable State in Lactobacillus delbrueckii subsp.bulgaricus ND02
摘要
Abstract
To improve the activity of lactic acid bacterial starter cultures during their production and application,we investigated the induction and resuscitation of viable but non-culturable (VBNC) state in Lactobacillus delbrueckii subsp.bulgaricus ND02 (Lactobacillus bulgaricus ND02),which had excellent fermentation characteristics.Lactobacillus bulgaricus ND02 was cultured under different conditions to induce the VBNC state.The plate count method and fluorescence microscope were used jointly to examine the VBNC state,and then the VBNC cells were resuscitated by varying temperature and medium components.The results showed that Lactobacillus bulgaricus ND02 in MRS at 4 ℃ could enter the VBNC state after 190 days.It was also found that skim milk (10％) plus yeast powder (0.1％) was effective for resuscitation.This study would provide a reference basis to prevent Lactobacillus bulgaricus ND02 from entering the VBNC state and thus to improve its activity for future work.关键词
保加利亚乳杆菌ND02/VBNC态/诱导/复苏Key words
Lactobacillus bulgaricus ND02/viable but nonculturable (VBNC)/induction/resuscitation分类
轻工纺织引用本文复制引用
王亚利,包秋华,王俊国,张和平..德氏乳杆菌保加利亚亚种ND02活的非可培养态诱导和复苏[J].食品科学,2017,38(6):68-73,6.基金项目
国家自然科学基金青年科学基金项目(31301516) （31301516）
内蒙古博士科研启动基金项目(BJ2013D-18) （BJ2013D-18）
内蒙古自然科学基金项目(2015MS0306) （2015MS0306）
