食品科学2017，Vol.38Issue(6)：88-95,8.DOI:10.7506/spkx1002-6630-201706014
双酶法制备马鹿茸降血糖肽工艺优化及其对α-葡萄糖苷酶的抑制效果
Optimization of Preparation of Hypoglycemic Peptides from Red Deer (Cervus elaphus) Antlers by Two-Step Enzymatic Hydrolysis and Their α-Glucosidase Inhibitory Activity
摘要
Abstract
This study was designed to optimize the enzymatic preparation of hypoglycemic peptides from red deer (Cervus elaphus) antlers.Alcalase,flavourzyme,neutral protcase and trypsin were comparatively evaluated based on α-glucosidase inhibitory activity of hydrolysates to select two suitable enzymes for sequential hydrolysis of red deer antlers.The sequences of two hydrolysis steps were compared for better hypoglycemic activity in vitro and the hydrolysis conditions were optimized based on degree of hydrolysis (DH),α-glucosidase inhibitory activity and protein recovery with one-factor-at-a-time method and orthogonal array design.The results showed that alcalase and flavourzyme were found to be more suitable and sequential use of alcalase followed by flavourzyme was optimal for higher percentage inhibition of α-glucosidase activity,DH and protein recovery,reaching 21.11％,19.88 ％ and 39.12％,respectively.The optimal hydrolysis conditions were obtained as follows.Red deer antlers were hydrolyzed firstly with 5 000 U/g alcalase at an initial pH of 8.0 and 60 ℃ with a substrate concentration of 12％ for 3 h,and then with 6 000 U/g flavourzyme for 1 h at 45 ℃ with a substrate concentration of 5％ after pH adjustment to 6.5.The final hydrolysate exhibited concentration-dependent α-glucosidase inhibitory activity,with a percentage inhibition of 94.09％ at 3 mg/rnL and IC50 value of 1.82 mg/mL.It was concluded that alcalase-flavourzyme hydrolysis would provide an efficient way to prepare hypoglycemic peptides from red deer antlers.关键词
马鹿茸/酶解/降血糖肽Key words
Cervus elaphus antler/enzymatic hydrolysis/hypoglycemic peptide分类
化学化工引用本文复制引用
包美丽,杨添植,张立钢,赵玉红..双酶法制备马鹿茸降血糖肽工艺优化及其对α-葡萄糖苷酶的抑制效果[J].食品科学,2017,38(6):88-95,8.基金项目
中央高校基本科研业务费专项(2572014CAQ02) （2572014CAQ02）
黑龙江省高校科技成果产业化前期研发培育项目(1254CGZH14) （1254CGZH14）
