食品科学2017，Vol.38Issue(6)：117-122,6.DOI:10.7506/spkx1002-6630-201706018
响应面试验优化微波辅助固定化胰蛋白酶工艺
Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology
摘要
Abstract
Trypsin was immobilized onto epoxy resin under microwave irradiation.Using one-factor-at-a-time method and response surface methodology (RSM),the optimal conditions of immobilization were obtained as follows:trypsin dosage,41 mg/g;microwave irradiation time,10 min;pH,6.2;and temperature,30 ℃.Under these conditions,the activity of immobilized trypsin was 322.56 U/g resin,which was higher than that of traditional immobilized trypsin and the immobilization time was shortened from 20 h to a few minutes.Furthermore,immobilized trypsin had a good stability.Thus,microwave-assisted immobilization provides an efficient way of immobilizing trypsin.关键词
固定化酶/环氧树脂/微波/响应面/高效Key words
immobilized trypsin/epoxy resin/microwave/response surface methodology/high efficiency分类
轻工纺织引用本文复制引用
刘伟华,李荣,姜子涛..响应面试验优化微波辅助固定化胰蛋白酶工艺[J].食品科学,2017,38(6):117-122,6.基金项目
天津市自然科学基金重点项目(12JCZDJC34100) （12JCZDJC34100）
天津市高等学校创新团队培养计划项目(TD12-5049) （TD12-5049）
