食品科学2017，Vol.38Issue(6)：142-146,5.DOI:10.7506/spkx1002-6630-201706022
黑曲霉N5-5单宁酶的纯化及酶学性质测定
Purification and Enzymatic Properties of Tannase from Aspergillus niger N5-5
摘要
Abstract
The crude tannase from Aspergillus niger N5-5 was purified by hollow fiber membrane ultrafiltration and Sephadex G-150 gel chromatography.The properties of the purified tannase were then determined.The results showed that the tannase from Aspergillus niger N5-5 could be purified about 20 folds with a 23.30％ recovery.The enzyme was a 64.2 kD protein with a single peptide chain by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.The optimal temperature for the enzyme was 45 ℃,and it had good thermostability in the range of 25-45 ℃;the optirnal pH value was 5.0,and the enzyme displayed good pH stability in the pH range of 5.0-5.5.In addition,the results of reaction kinetics showed that the Km and vmax values towards the substrate propyl gallate were 0.916 mmol/L and 0.877 mmol/(L·min),respectively.关键词
单宁酶/黑曲霉/纯化/纯酶/性质测定Key words
tannase/Aspergillus niger/purification/purified enzyme/characterization分类
化学化工引用本文复制引用
张帅,曹庸,梁晓莹,林婉如..黑曲霉N5-5单宁酶的纯化及酶学性质测定[J].食品科学,2017,38(6):142-146,5.基金项目
广东高校国际科技合作创新平台项目(2013gjhz0003) （2013gjhz0003）
